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Chung-Der Hsiao

2012-05-04
17:00:00 - 18:00:00

103 , Mathematics Research Center Building (ori. New Math. Bldg.)

Fish scale cover and protect the body from injury. Fish scale is a dermis-derived structure which covered by skin and constitute majorly by collagen and calcium. Fish scale contains osteoblasts, osteoclasts, and bone matrix, all of which are similar to those found in mammals bones. The specific aim of this research is to analyze the scale regeneration process at histological and molecular levels. To archive this goal, we created Tg(osx:nlsGFP)cy25 to illuminate scale and bone. After scale amputation, the regenerating scale display strong green fluorescence and served as a good living model to study scale regeneration in vivo. Combination of histological and morphological observation, five major events of scale regeneration as follows: (1) 0-2 dpa (days-post amputation): wound healing go first and later follow by mesenchymal condensation. (2) 4 dpa: scale reaches 50% regeneration, protrudes out the placode and star to mineralization from 4 dpa onwards. (3) 12dpa: scale reaches 90% regeneration and gradually down-regulated regeneration activity. (4) 20 dpa: scale regeneration complete. Next, we used microarray to explore the gene expression profiling at specific time points. Results show some genes involved in osteogenesis and osteoclastogenesis are highly expressed accompanying with the regeneration climax. This result shows the gene expression profiling during scale regeneration is similar to mammal bone growth. In conclusion, we suggest zebrafish scale regeneration process might be served as an alternative model to explore gene expression and function of human bone growth and repair. This is a joint work with Ting-Cih Huang (Department of Bioscience Technology, Chung Yuan Christian University) and Hsin-Yu Lee (Institute of Zoology, National Taiwan University)